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KMID : 0381120120340010027
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Genes and Genomics
2012 Volume.34 No. 1 p.27 ~ p.34
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Effect of purine limitation caused by an amidophosphoribosyl transferase (purF) mutation on polyphosphate kinase 1 (ppk1) gene expression
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Jung Sam-Il
Lee Myeong-Sok
Kim Soo-Ki
Wanner Barry L.
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Abstract
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Polyphosphate kinase (ppk) synthesizes polyphosphate (polyPn+1) from polyPn and ATP and also catalyses its reversal reaction. In the process of identifying stimuli that affect E. coli ppk transcription, we show that purine limitation activates the E. coli ppk promoter by using a single-copy E. coli ppk promoter-lacZ (ppkP Ec -lacZ) fusion and Ez-Tn5 random mutagenesis. The ppkP Ec -lacZ expression was greatly induced (seven to eleven fold higher depending on the media) in purF mutant cells (e.g. cvpA::Ez-Tn5 and ΔpurF mutants). This behavior seems to result from the purine starvation because the ppkP Ec -lacZ expression in the purF mutant cells was recovered to the level of wild-type cells by the addition of any type of purines (adenine, guanine, adenosine, and guanosine). The ppkP Ec -lacZ expression was also increased in other pur mutants but not as much as in the purF mutant cells. Interestingly, the ppkP Ec -lacZ expression was not changed in mutants with defective pyrimidine specific genes (e.g. ΔpyrE and ΔthiC). Transcription of four other bacterial ppk promoters also increased in the purF mutant cells. This data imply that purine deficiency seems to be a common and good inducer in bacterial ppk gene expression. Here, we present a novel report about the effect of purine biosynthesis on ppk gene expression in bacteria.
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KEYWORD
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Polyphosphate kinase, Purine, ppk gene expression
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